Ephrin B1 antibody (pSer300) (AbBy Fluor® 594)

Catalog No. ABIN2812863

This Rabbit Polyclonal antibody specifically detects Ephrin B1 in WB and IF (p). It exhibits reactivity toward Human, Mouse, Rat and Cow.

Quick Overview for Ephrin B1 antibody (pSer300) (AbBy Fluor® 594) (ABIN2812863)

Target: Ephrin B1 (EFNB1)

Reactivity: Human, Mouse, Rat, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ephrin B1 antibody is conjugated to AbBy Fluor® 594

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-Ephrin B1 Antibody (AbBy Fluor® 594)

Binding Specificity: pSer300

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Ephrin B around the phosphorylation site of Ser300

Isotype: IgG

Application Details

Application Notes: IF(IHC-P)(1:50-200)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Ephrin B1

Target: Ephrin B1 (EFNB1)

Alternative Name: EFNB1

Background:

Synonyms: Ephrin B phospho Y330, p-Ephrin B phospho Y330, CEK5 ligand, EFNB1, ELK L, EphrinB, Ephrin-B, ELK ligand, ELKL, EPH related receptor tyrosine kinase ligand 2, Ephrin B Precursor, Ephrin B1, Ephrin B2, LERK 2, LERK2, EFNB2_HUMAN.

Background: Ephrin B proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development. Ephrin B molecules expressed at the membrane surface bind to the Ephrin B family receptors on target cells during cell to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing Ephrin B on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of Ephrin B is thought to be a critical component of this reverse signaling event. Recent work demonstrated that Tyr331 of Ephrin B was phosphorylated in HEK293 cells after stimulation by the soluble Ephrin B2 receptor tyrosine kinase.

Gene ID: 1947

Pathways: RTK Signaling