FYB antibody (AA 501-600) (AbBy Fluor® 594)

Catalog No. ABIN2813440

The Rabbit Polyclonal anti-FYB antibody has been validated for IF (cc) and IF (p). It is suitable to detect FYB in samples from Human.

Quick Overview for FYB antibody (AA 501-600) (AbBy Fluor® 594) (ABIN2813440)

Target: FYB (FYN-Binding Protein (FYB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FYB antibody is conjugated to AbBy Fluor® 594

Application: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-FYB Antibody (AbBy Fluor® 594)

Binding Specificity: AA 501-600

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human FYB/ADAP

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for FYB

Target: FYB (FYN-Binding Protein (FYB))

Alternative Name: FYB/ADAP

Background:

Synonyms: ADAP, Adhesion and degranulation promoting adaptor protein, FYB 120/130, Fyb, FYB-120/130, FYB_HUMAN, FYN binding protein, FYN T binding protein, FYN-binding protein, FYN-T-binding protein, p120/p130, PRO0823, SLAP 130, SLAP-130, SLAP130, SLP 76 associated phosphoprotein, SLP-76-associated phosphoprotein, SLP76 associated phosphoprotein.

Background: Fyb (Fyn binding protein) and the anchoring proteins SKAP55 (src kinase-associated phosphoprotein) and SKAP55-R (SKAP55-related protein) associate with the tyrosine kinase p59fyn (13). SKAP55 and SKAP55-R bind to Fyb through their SH3 domains and function as substrates for p59Fyn in resting T cells (13). SKAP55 contains an amino-terminal pleckstrin homology domain and a carboxy-terminal SH3 domain binding motif of adjacent arginine and lysine residues followed by tandem tyrosines (i.e. RKxxYxxY) (4,5). SKAP55-R, similar in overall structure to SKAP55, contains a coiled-coil N-terminal domain (1,2). SKAP55 associates with SLAP-130, another component of the Fyn complex, which plays a role in the regulation of signaling events initiated by lymphocyte antigen receptors leading up to T cell activation (6). The human Fyb gene maps to chromosome 5p13.1 and encodes a 783 amino acid protein (7).

Gene ID: 2533

Pathways: TCR Signaling, Protein targeting to Nucleus