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Nestin antibody (AA 1389-1416)

This anti-Nestin antibody is a Rabbit Polyclonal antibody detecting Nestin in WB, IF, FACS and IHC (p). Suitable for Human. This Primary Antibody has been cited in 1 publication.
Catalog No. ABIN2839051

Quick Overview for Nestin antibody (AA 1389-1416) (ABIN2839051)

Target

See all Nestin (NES) Antibodies
Nestin (NES)

Reactivity

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  • 1
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Human

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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  • 1
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This Nestin antibody is un-conjugated

Application

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Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone

RB16253
  • Binding Specificity

    • 16
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    AA 1389-1416

    Purification

    This antibody is purified through a protein A column, followed by peptide affinity purification.

    Immunogen

    This Nestin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1389-1416 amino acids from human Nestin.

    Isotype

    Ig Fraction
  • Application Notes

    IF: 1:200. IF: 1:25. WB: 1:2000. WB: 1:2000. IHC-P: 1:25. IHC-P: 1:25. IHC-P: 1:25

    Restrictions

    For Research Use only
  • Format

    Liquid

    Buffer

    Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

    Expiry Date

    6 months
  • He, Qiu, Wang, Gui, Wang, Hu, Xia, Qi, Wu, Ma: "Multivoxel magnetic resonance spectroscopy identifies enriched foci of cancer stem-like cells in high-grade gliomas." in: OncoTargets and therapy, Vol. 10, pp. 195-203, (2017) (PubMed).

  • Target

    Nestin (NES)

    Alternative Name

    Nestin

    Background

    Nestin is a class VI intermediate filament protein expressed predominantly in stem cells of the neural tube but absent from virtually all differentiated CNS cells. In the CNS, nestin is downregulated upon differentiation and replaced by neurofilaments. Transient expression of nestin has been postulated as a key step committing cells to the neural differentiation pathway. Nestin expression has also been observed in pancreatic hematopoietic stem cell populations.

    Molecular Weight

    177439

    Gene ID

    10763

    NCBI Accession

    NP_006608

    UniProt

    P48681
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