USP2 antibody (N-Term)

Catalog No. ABIN2839170

The Rabbit Polyclonal anti-USP2 antibody has been validated for WB and IHC (p). It is suitable to detect USP2 in samples from Human and Rat. There are 4+ publications available.

Quick Overview for USP2 antibody (N-Term) (ABIN2839170)

Target: USP2 (Ubiquitin Specific Peptidase 2 (USP2))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB4202

Product Details anti-USP2 Antibody

Binding Specificity: AA 71-101, N-Term

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This USP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 71-101 amino acids from the N-terminal region of human USP2.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000. WB: 1:1000. IHC-P: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-USP2 antibody (ABIN2839170)

Kitamura, Ishino, Shimamoto, Okabe, Miyamoto, Takahashi, Miyoshi: "Ubiquitin-Specific Protease 2 Modulates the Lipopolysaccharide-Elicited Expression of Proinflammatory Cytokines in Macrophage-like HL-60 Cells." in: Mediators of inflammation, Vol. 2017, pp. 6909415, (2018) (PubMed).

Kitamura, Kimura, Shimamoto, Okabe, Ito, Miyamoto, Naoe, Kikuguchi, Meek, Toda, Okamoto, Kanehira, Hase, Watarai, Ishizuka, El-Osta, Ohara, Miyoshi: "Ubiquitin-specific protease 2-69 in macrophages potentially modulates metainflammation." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 27, Issue 12, pp. 4940-53, (2013) (PubMed).

Stevenson, Sparks, Allende-Vega, Xirodimas, Lane, Saville: "The deubiquitinating enzyme USP2a regulates the p53 pathway by targeting Mdm2." in: The EMBO journal, Vol. 26, Issue 4, pp. 976-86, (2007) (PubMed).

Priolo, Tang, Brahamandan, Benassi, Sicinska, Ogino, Farsetti, Porrello, Finn, Zimmermann, Febbo, Loda: "The isopeptidase USP2a protects human prostate cancer from apoptosis." in: Cancer research, Vol. 66, Issue 17, pp. 8625-32, (2006) (PubMed).

Target Details for USP2

Target: USP2 (Ubiquitin Specific Peptidase 2 (USP2))

Alternative Name: USP2

Background: Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

Molecular Weight: 68072

Gene ID: 9099

NCBI Accession: NP_001230688, NP_004196, NP_741994

UniProt: O75604