LLGL1+2 (pSer650), (pSer654) antibody

Catalog No. ABIN388990

This Rabbit Polyclonal antibody specifically detects LLGL1+2 in WB. It exhibits reactivity toward Human.It has been mentioned in 1 publication

Quick Overview for LLGL1+2 (pSer650), (pSer654) antibody (ABIN388990)

Target: LLGL1+2

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Clone: RB7629-RB7630

Product Details

Binding Specificity: pSer650, pSer654

Predicted Reactivity: Cow (Bovine),Mouse (Murine),Rat (Rattus)

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This LLGL1/2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S645/S649 of human LLGL1/2.

Isotype: Ig Fraction

Application Details

Application Notes: Suggested dilutions:
WB = 1:1000
Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Expiry Date: 6 months

References

Solinet, Akpovi, Garcia, Barry, Vitale: "Myosin IIB deficiency in embryonic fibroblasts affects regulators and core members of the par polarity complex." in: Histochemistry and cell biology, Vol. 136, Issue 3, pp. 245-66, (2011) (PubMed).

Target Details

Target: LLGL1+2

Background: Function
Part of a complex with GPSM2/LGN, PRKCI/aPKC and PARD6B/Par-6, which may ensure the correct organization and orientation of bipolar spindles for normal cell division. This complex plays roles in the initial phase of the establishment of epithelial cell polarity.

Cellular Location
Cytoplasm. Note=Localized in the perinuclear structure and faintly at the cell-cell contacts sites in the interphase. Localized at the cell periphery during metaphase Cortical localization in mitotic cells. Found in the lateral region of polarized epithelial cells

Synonyms: Lethal(2) giant larvae protein homolog 2, HGL, LLGL2, Lethal(2) giant larvae protein homolog 1, LLGL, Human homolog to the D-lgl gene, Hugl-1, DLG4, LLGL1, HUGL

Molecular Weight: 115042 Da

Gene ID: 3993

UniProt: Q6P1M3