RAD9A antibody (full length)

Catalog No. ABIN363404

The Rabbit Polyclonal anti-RAD9A antibody has been validated for WB, IF and ICC. It is suitable to detect RAD9A in samples from Human.

Quick Overview for RAD9A antibody (full length) (ABIN363404)

Target: RAD9A (RAD9 Homolog A (S. Pombe) (RAD9A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD9A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-RAD9A Antibody

Binding Specificity: full length

Specificity: Reacts with human RAD9

Cross-Reactivity (Details): Not tested in other species.

Purification: Antigen affinity purified

Immunogen: Fusion protein of full length human RAD9 protein

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
- ICC/IF : 1/500 - WB : 1/100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: , Sodium azide 0.1 %

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Short term storage at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.

Expiry Date: 6 months

Target Details for RAD9A

Target: RAD9A (RAD9 Homolog A (S. Pombe) (RAD9A))

Alternative Name: RAD9

Background: Rad9 (cell cycle checkpoint control protein RAD9A or hRAD9) is an evolutionarily conserved protein which serves multiple functions for preserving genomic integrity, and besides its role as intra-S/G2 checkpoint , it also implicates in homologous recombination repair, base excision repair and mismatch repair pathways. PRKCD mediated Rad9 phosphorylation is essential for the assembly of 9-1-1 cell-cycle checkpoint response complex which is recruited to DNA lesion upon damage by RFC (RAD17-replication factor C) clamp loader complex. Thereafter, Rad9 acts as sliding clamp platform on DNA for multiple proteins involved in LP-BER (long-patch base excision repair). 9-1-1 complex stimulates DNA polymerase beta activity/stability, and is necessary for the recruitment of RHNO1 to sites of DNA-DSBs that occurs during S phase of cell cycle. Moreover, Rad9 exerts 3'->5' double stranded DNA exonuclease activity, and it transactivate p53 target genes such as p21 and has been shown to induce apoptosis through its interaction with Bcl-2/Bcl-xL.

UniProt: Q99638

Pathways: Positive Regulation of Response to DNA Damage Stimulus