PLAU antibody (N-Term)

Catalog No. ABIN2853629

The Rabbit Polyclonal anti-PLAU antibody is suitable to detect PLAU in samples from Human and Mouse. It has been validated for WB, ELISA and IHC (p).

Quick Overview for PLAU antibody (N-Term) (ABIN2853629)

Target: PLAU (Plasminogen Activator, Urokinase (PLAU))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLAU antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PLAU Antibody

Binding Specificity: AA 68-98, N-Term

Specificity: This antibody recognizes Urokinase/PLAU (N-term).

Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between aa 68-98 from the N-terminal region of human PLAU.

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/100-1/500. Immunohistochemistry: 1/50-1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09% (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.

Expiry Date: 12 months

Target Details for PLAU

Target: PLAU (Plasminogen Activator, Urokinase (PLAU))

Alternative Name: uPA / PLAU

Background: PLAU, a member of the peptidase family S1, is a potent plasminogen activator and is clinically used for therapy of thrombolytic disorders. PLAU specifically cleaves the Arg-|-Val bond in plasminogen to form plasmin. The protein is found in high and low molecular mass forms. Each consists of two chains, A and B. The high molecular mass form contains a long chain A. Cleavage occurs after residue 155 in the low molecular mass form to yield a short A1 chain. The protein is used in Pulmonary Embolism (PE) to initiates fibrinolysis. Structurally, PLAU contains 1 EGF-like domain and 1 kringle domain.
Alternate names: U-plasminogen activator, Urokinase, Urokinase-type plasminogen activator, Urokinase- type plasminogen activator chain B, Urokinase-type plasminogen activator long chain A, Urokinase-type plasminogen activator short chain A

Gene ID: 5328, 9606

UniProt: P00749

Pathways: Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration