Ataxin 3 antibody

Catalog No. ABIN2854322

The Rabbit Polyclonal anti-Ataxin 3 antibody has been validated for WB, IF, IP and ICC. It is suitable to detect Ataxin 3 in samples from Human.

Quick Overview for Ataxin 3 antibody (ABIN2854322)

Target: Ataxin 3 (ATXN3)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ataxin 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Grade: KO Validated

Product Details anti-Ataxin 3 Antibody

Cross-Reactivity: Human

Characteristics: Rabbit Polyclonal antibody to Ataxin 3 (ataxin-3)
Ataxin 3 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Ataxin 3. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: SK-N-SH , SK-N-AS , human Ataxin3-transfected 293T , 293T , A431 , HeLa , HepG2

Validation: KO/KD, Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.55 mg/mL

Buffer: 1XPBS ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Ataxin 3

Target: Ataxin 3 (ATXN3)

Alternative Name: ataxin 3

Background: Machado-Joseph disease, also known as spinocerebellar ataxia-3, is an autosomal dominant neurologic disorder. The protein encoded by this gene contains (CAG)n repeats in the coding region, and the expansion of these repeats from the normal 13-36 to 68-79 is the cause of Machado-Joseph disease. There is a negative correlation between the age of onset and CAG repeat numbers. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Cellular Localization: Nucleus matrix

Molecular Weight: 42 kDa

Gene ID: 4287

UniProt: P54252