Septin 2 antibody

Catalog No. ABIN2854352

The Rabbit Polyclonal anti-Septin 2 antibody has been validated for WB and IHC (p). It is suitable to detect Septin 2 in samples from Human.

Quick Overview for Septin 2 antibody (ABIN2854352)

Target: Septin 2 (SEPT2)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Septin 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Septin 2 Antibody

Cross-Reactivity: Human

Characteristics: Rabbit Polyclonal antibody to Septin 2 (septin 2)
Septin 2 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Septin 2. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: U87-MG , SK-N-SH

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Septin 2

Target: Septin 2 (SEPT2)

Alternative Name: septin 2

Background: Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Plays a role in the biogenesis of polarized columnar-shaped epithelium by maintaining polyglutamylated microtubules, thus facilitating efficient vesicle transport, and by impeding MAP4 binding to tubulin. Required for the progression through mitosis. Forms a scaffold at the midplane of the mitotic splindle required to maintain CENPE localization at kinetochores and consequently chromosome congression. During anaphase, may be required for chromosome segregation and spindle elongation. May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri.

Cellular Localization: Cytoplasm , Spindle

Molecular Weight: 41 kDa

Gene ID: 4735

UniProt: Q15019