E2F3 antibody

Catalog No. ABIN2854385

Product Details anti-E2F3 Antibody

Target: E2F3

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F3 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Knockdown/Knockout validation was supported by customer review data.

Cross-Reactivity: Human

Characteristics: Rabbit Polyclonal antibody to E2F3 (E2F transcription factor 3)
E2F3 antibody [N2C3]

Purification: Purified by antigen-affinity chromatography.

Grade: KO Validated

Immunogen: Recombinant protein encompassing a sequence within the center region of human E2F3. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.28 mg/mL

Buffer: 1XPBS ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for E2F3

Target: E2F3

Alternative Name: E2F transcription factor 3 (E2F3 Products)

Background: The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F2, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner.

Molecular Weight: 49 kDa

Gene ID: 1871

UniProt: O00716