E2F3 antibody (AA 381-465)

Catalog No. ABIN733493

There are 2+ publications for this product available. The Rabbit Polyclonal anti-E2F3 antibody is suitable to detect E2F3 in samples from Human. It has been validated for WB, IF (cc), IF (p), IHC (p) and IHC (fro).

Quick Overview for E2F3 antibody (AA 381-465) (ABIN733493)

Target: E2F3

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-E2F3 Antibody

Binding Specificity: AA 381-465

Purpose: E2F3 Polyclonal Antibody

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human E2F3

Isotype: IgG

Application Details

Application Notes: WB(1:300-5000),

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-E2F3 antibody (ABIN733493)

Yang, Wang, Tang, Bai: "miR-203a suppresses cell proliferation by targeting E2F transcription factor 3 in human gastric cancer." in: Oncology letters, Vol. 14, Issue 6, pp. 7687-7690, (2017) (PubMed).

Target Details for E2F3

Target: E2F3

Background:

Synonyms: E2F-3, Transcription factor E2F3, E2F3, KIAA0075

Background: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner. Inhibits adipogenesis, probably through the repression of CEBPA binding to its target gene promoters (By similarity).

Gene ID: 1871

UniProt: O00716