E2F3 antibody (AA 381-465)

Catalog No. ABIN733493

Product Details anti-E2F3 Antibody

Target: E2F3

Binding Specificity: AA 381-465

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Dog,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human E2F3

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-E2F3 antibody (ABIN733493)

Yang, Wang, Tang, Bai: "miR-203a suppresses cell proliferation by targeting E2F transcription factor 3 in human gastric cancer." in: Oncology letters, Vol. 14, Issue 6, pp. 7687-7690, (2017) (PubMed).

Target Details for E2F3

Target: E2F3

Abstract: E2F3 Products

Synonyms: E2F-3 antibody, E2F3b antibody, E2f3a antibody, mKIAA0075 antibody, RGD1561600 antibody, E2F transcription factor 3 antibody, E2F transcription factor 3 S homeolog antibody, E2F3 antibody, e2f3.S antibody, E2f3 antibody

Background:

Synonyms: E2F-3, Transcription factor E2F3, E2F3, KIAA0075

Background: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner. Inhibits adipogenesis, probably through the repression of CEBPA binding to its target gene promoters (By similarity).

Gene ID: 1871

UniProt: O00716