HAAO antibody

Catalog No. ABIN2854465

This Rabbit Polyclonal antibody specifically detects HAAO in WB. It exhibits reactivity toward Human.

Quick Overview for HAAO antibody (ABIN2854465)

Target: HAAO (3-hydroxyanthranilate 3,4-Dioxygenase (HAAO))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HAAO antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HAAO Antibody

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to HAAO (3-hydroxyanthranilate 3,4-dioxygenase)
HAAO antibody [N1C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human HAAO. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:10000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Mouse liver , HepG2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for HAAO

Target: HAAO (3-hydroxyanthranilate 3,4-Dioxygenase (HAAO))

Alternative Name: 3-hydroxyanthranilate 3,4-dioxygenase

Background: 3-Hydroxyanthranilate 3,4-dioxygenase is a monomeric cytosolic protein belonging to the family of intramolecular dioxygenases containing nonheme ferrous iron. It is widely distributed in peripheral organs, such as liver and kidney, and is also present in low amounts in the central nervous system. HAAO catalyzes the synthesis of quinolinic acid (QUIN) from 3-hydroxyanthranilic acid. QUIN is an excitotoxin whose toxicity is mediated by its ability to activate glutamate N-methyl-D-aspartate receptors. Increased cerebral levels of QUIN may participate in the pathogenesis of neurologic and inflammatory disorders. HAAO has been suggested to play a role in disorders associated with altered tissue levels of QUIN.

Cellular Localization: Cytoplasm

Molecular Weight: 33 kDa

Gene ID: 23498

UniProt: P46952