LGI1 antibody

Catalog No. ABIN2854526

The Rabbit Polyclonal anti-LGI1 antibody has been validated for WB, IF, ICC. It is suitable to detect LGI1 in samples from Human.

Quick Overview for LGI1 antibody (ABIN2854526)

Target: LGI1 (Leucine-Rich, Glioma Inactivated 1 (LGI1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LGI1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-LGI1 Antibody

Cross-Reactivity: Human, Rat

Characteristics: Rabbit polyclonal antibody to LGI1 (leucine-rich, glioma inactivated 1)
LGI1 antibody [N2C2], Internal

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human LGI1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A431 , U87-MG , SK-N-SH , SK-N-AS , rat brain

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for LGI1

Target: LGI1 (Leucine-Rich, Glioma Inactivated 1 (LGI1))

Alternative Name: leucine rich glioma inactivated 1

Background: This gene is rearranged as a result of translocations in glioblastoma cell lines. The protein contains a hydrophobic segment representing a putative transmembrane domain with the amino terminus located outside the cell. It also contains leucine-rich repeats with conserved cysteine-rich flanking sequences. This gene is predominantly expressed in neural tissues and its expression is reduced in low grade brain tumors and significantly reduced or absent in malignant gliomas. Mutations in this gene result in autosomal dominant lateral temporal epilepsy.

Cellular Localization: Secreted

Molecular Weight: 64 kDa

Gene ID: 9211

UniProt: O95970