STAU1/Staufen antibody (C-Term)

Catalog No. ABIN2854568

The Rabbit Polyclonal anti-STAU1/Staufen antibody has been validated for WB and IP. It is suitable to detect STAU1/Staufen in samples from Human and Mouse.

Quick Overview for STAU1/Staufen antibody (C-Term) (ABIN2854568)

Target: STAU1/Staufen (STAU1) (Staufen Double-Stranded RNA Binding Protein 1 (STAU1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STAU1/Staufen antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-STAU1/Staufen Antibody

Binding Specificity: C-Term

Cross-Reactivity: Rhesus Monkey

Cross-Reactivity (Details): Rhesus Monkey (100 %)

Characteristics: Rabbit Polyclonal antibody to Staufen (staufen, RNA binding protein, homolog 1 (Drosophila))
Staufen antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human Staufen. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference Immunoprecipitation 1:500-1:3000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceImmunoprecipitation1:500-1:3000* Western blot1:500-1:3000*

Comment:

Positive Control: 293T , A431 , H1299 , HepG2 , Molt-4 , Raji , Neuro2A , 293 (input) , Stau1-IP (GTX106566) , Post-IP lysate from control rabbit IgG-IP , Post-IP lysate from Stau1-IP

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.73 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for STAU1/Staufen

Target: STAU1/Staufen (STAU1) (Staufen Double-Stranded RNA Binding Protein 1 (STAU1))

Alternative Name: Staufen

Background: Staufen is a member of the family of double-stranded RNA (dsRNA)-binding proteins involved in the transport and/or localization of mRNAs to different subcellular compartments and/or organelles. These proteins are characterized by the presence of multiple dsRNA-binding domains which are required to bind RNAs having double-stranded secondary structures. The human homologue of staufen encoded by STAU, in addition contains a microtubule- binding domain similar to that of microtubule-associated protein 1B, and binds tubulin. The STAU gene product has been shown to be present in the cytoplasm in association with the rough endoplasmic reticulum (RER), implicating this protein in the transport of mRNA via the microtubule network to the RER, the site of translation. Five transcript variants resulting from alternative splicing of STAU gene and encoding three isoforms have been described. Three of these variants encode the same isoform, however, differ in their 5'UTR.

Cellular Localization: Rough endoplasmic reticulum

Molecular Weight: 63 kDa

Gene ID: 6780

Pathways: Asymmetric Protein Localization