Caveolin-1 antibody (N-Term)

Catalog No. ABIN2854651

This Rabbit Polyclonal antibody specifically detects Caveolin-1 in WB, IF, IP, ICC and IHC (p). It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for Caveolin-1 antibody (N-Term) (ABIN2854651)

Target: Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caveolin-1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: KO Validated

Product Details anti-Caveolin-1 Antibody

Binding Specificity: N-Term

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit polyclonal antibody to Caveolin 1 (caveolin 1, caveolae protein, 22 kDa)
Caveolin 1 antibody [N1N3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Caveolin 1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A431 , H1299 , HeLa , mouse lung , HeLa

Validation: KO/KD, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-Caveolin-1 antibody (ABIN2854651)

Fang, Lin, Liang, Liang: "A novel c-Kit/phospho-prohibitin axis enhances ovarian cancer stemness and chemoresistance via Notch3-PBX1 and β-catenin-ABCG2 signaling." in: Journal of biomedical science, Vol. 27, Issue 1, pp. 42, (2020) (PubMed).

Target Details for Caveolin-1

Target: Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

Alternative Name: caveolin 1

Background: The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 MAP kinase cascade. CAV1 and CAV2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. By using alternative initiation codons in the same reading frame, two isoforms (alpha and beta) are encoded by a single transcript from this gene.

Cellular Localization: Golgi apparatus membrane , Cell membrane , Membrane , caveola

Molecular Weight: 20 kDa

Gene ID: 857

UniProt: Q03135

Pathways: Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of Transporter Activity, VEGFR1 Specific Signals