HTR2C antibody (C-Term)

Catalog No. ABIN2854715

The Rabbit Polyclonal anti-HTR2C antibody has been validated for WB, IHC (p) and IHC (fro). It is suitable to detect HTR2C in samples from Human.

Quick Overview for HTR2C antibody (C-Term) (ABIN2854715)

Target: HTR2C (5-Hydroxytryptamine (serotonin) Receptor 2C (HTR2C))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HTR2C antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-HTR2C Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to 5-HT2C receptor
5-HT2C receptor antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human 5-HT2C receptor. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: rat brain , mouse placenta

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.68 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for HTR2C

Target: HTR2C (5-Hydroxytryptamine (serotonin) Receptor 2C (HTR2C))

Alternative Name: 5-hydroxytryptamine receptor 2C

Background: Serotonin (5-hydroxytryptamine, 5-HT), a neurotransmitter, elicits a wide array of physiological effects by binding to several receptor subtypes, including the 5-HT2 family of seven-transmembrane-spanning, G-protein-coupled receptors, which activate phospholipase C and D signaling pathways. This gene encodes the 2C subtype of serotonin receptor and its mRNA is subject to multiple RNA editing events, where genomically encoded adenosine residues are converted to inosines. RNA editing is predicted to alter amino acids within the second intracellular loop of the 5-HT2C receptor and generate receptor isoforms that differ in their ability to interact with G proteins and the activation of phospholipase C and D signaling cascades, thus modulating serotonergic neurotransmission in the central nervous system. Studies in humans have reported abnormalities in patterns of 5-HT2C editing in depressed suicide victims.

Cellular Localization: Cell membrane

Molecular Weight: 52 kDa

Gene ID: 3358

UniProt: P28335

Pathways: Inositol Metabolic Process, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour