UBE2B antibody

Catalog No. ABIN2854744

This Rabbit Polyclonal antibody specifically detects UBE2B in WB, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for UBE2B antibody (ABIN2854744)

Target: UBE2B (Ubiquitin-Conjugating Enzyme E2B (UBE2B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-UBE2B Antibody

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to UBE2B (ubiquitin-conjugating enzyme E2 B)
UBE2B antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human UBE2B. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Mouse brain , 3xFlag-human UBE2B-transfected 293T

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.72 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for UBE2B

Target: UBE2B (Ubiquitin-Conjugating Enzyme E2B (UBE2B))

Alternative Name: ubiquitin conjugating enzyme E2 B

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100 % identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution.

Cellular Localization: Cell membrane (By similarity) , Nucleus (By similarity)

Molecular Weight: 17 kDa

Gene ID: 7320

UniProt: P63146