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EGFR antibody (C-Term)

Name: EGFR antibody (C-Term)
Brand: antibodies-online
SKU: ABIN2854754
Price: 637.78 USD
Availability: InStock

KO Validated EGFR Reactivity: Human WB, IF, FACS, IHC (p), IP, ICC Host: Rabbit Polyclonal unconjugated

WB Image Sample (30 ug of whole cell lysate) A: A431 5% SDS PAGE antibody diluted at 1:10000 (EGFR antibody (C-Term))

ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using EGFR, antibody (Green) at 1:1000 dilution. (EGFR antibody (C-Term))

IP Image EGFR antibody [C2C3], C-term immunoprecipitates EGFR protein in IP experiments. (EGFR antibody (C-Term))

IHC-P Image EGFR antibody [C2C3], C-term detects EGFR protein at membrane on human gastric cancer by immunohistochemical analysis. (EGFR antibody (C-Term))

IHC

5 images

Catalog No. ABIN2854754

Datasheet as PDF

Target See all EGFR Antibodies

EGFR (Epidermal Growth Factor Receptor (EGFR))
Binding Specificity
39

38

31

30

30

27

27

26

24

22

19

18

16

16

16

15

15

15

11

11

10

9

9

9

8

8

7

6

5

5

5

4

4

4

4

4

4

4

4

3

3

3

3

3

3

3

3

3

3

3
C-Term
Reactivity
747

296

283

25

25

24

19

14

8

3

2

2

1

1

1

1

1

1
Human
Host
579

167

14

13

5

2

1

1
Rabbit
Compatible Secondaries
Clonality
541

236

2
Polyclonal
Conjugate
409

58

29

22

19

17

16

16

16

16

16

11

10

10

10

10

10

10

10

10

10

8

8

5

4

4

4

4

4

1

1

1

1

1

1
This EGFR antibody is un-conjugated
Application
534

248

149

144

125

102

97

92

82

45

37

34

34

18

11

8

6

4

4

3

3

3

3

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Specificity

IP/MS validation was supported by references (PMID:30377372)

Cross-Reactivity

Human, Mouse, Rat

Characteristics

Rabbit Polyclonal antibody to EGFR (epidermal growth factor receptor)
EGFR antibody [C2C3], C-term

Purification

Purified by antigen-affinity chromatography.

Grade

KO Validated

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human EGFR. The exact sequence is proprietary.

Isotype

IgG

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Application Notes

WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment

Positive Control: A431
Validation: IP/MS, KO/KD, Orthogonal

Restrictions

For Research Use only
Format

Liquid

Concentration

0.28 mg/mL

Buffer

1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Target

EGFR (Epidermal Growth Factor Receptor (EGFR))

Alternative Name

epidermal growth factor receptor (EGFR Products)

Background

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.

Cellular Localization: Cell membrane, Single-pass type I membrane protein , Isoform 2: Secreted

Molecular Weight

134 kDa

Gene ID

1956

UniProt

P00533

Pathways

NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins