EGFR antibody (C-Term)

Catalog No. ABIN2854754

The Rabbit Polyclonal anti-EGFR antibody has been validated for WB, IF, FACS, IHC (p), IP and ICC. It is suitable to detect EGFR in samples from Human.

Quick Overview for EGFR antibody (C-Term) (ABIN2854754)

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EGFR antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Grade: KO Validated

Product Details anti-EGFR Antibody

Binding Specificity: C-Term

Specificity: IP/MS validation was supported by references (PMID:30377372)

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to EGFR (epidermal growth factor receptor)
EGFR antibody [C2C3], C-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human EGFR. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A431

Validation: IP/MS, KO/KD, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.28 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for EGFR

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Alternative Name: epidermal growth factor receptor

Background: The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.

Cellular Localization: Cell membrane, Single-pass type I membrane protein , Isoform 2: Secreted

Molecular Weight: 134 kDa

Gene ID: 1956

UniProt: P00533

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins