IGFBP3 antibody

Catalog No. ABIN2854756

This Rabbit Polyclonal antibody specifically detects IGFBP3 in WB, IHC (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for IGFBP3 antibody (ABIN2854756)

Target: IGFBP3 (Insulin-Like Growth Factor Binding Protein 3 (IGFBP3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IGFBP3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-IGFBP3 Antibody

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to IGFBP3 (insulin-like growth factor binding protein 3)
IGFBP3 antibody [N2C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human IGFBP3. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-Fr: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: mouse plasma

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for IGFBP3

Target: IGFBP3 (Insulin-Like Growth Factor Binding Protein 3 (IGFBP3))

Alternative Name: insulin like growth factor binding protein 3

Background: This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Cellular Localization: Secreted

Molecular Weight: 32 kDa

Gene ID: 3486

UniProt: P17936

Pathways: Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Growth Factor Binding