MMP 9 antibody

Catalog No. ABIN2854757

There are 5+ publications for this product available. The Rabbit Polyclonal anti-MMP 9 antibody is suitable to detect MMP 9 in samples from Human. It has been validated for WB, IHC, ICC, IF, IHC (p), IHC (fro) and IHC (wm).

Quick Overview for MMP 9 antibody (ABIN2854757)

Target: MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MMP 9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Whole Mount) (IHC (wm))

Product Details anti-MMP 9 Antibody

Cross-Reactivity: Cow, Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to MMP9 (matrix metallopeptidase 9 (gelatinase B, 92 kDa gelatinase, 92 kDa type IV collagenase))
MMP9 antibody [N2C1], Internal

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human MMP9. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.88 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-MMP 9 antibody (ABIN2854757)

Tsai, Kuo, Lin, Ho, Wang, Chuang, Liou: "Ischemic Stroke Risk Associated with Mitochondrial Haplogroup F in the Asian Population." in: Cells, Vol. 9, Issue 8, (2020) (PubMed).

Chung, Lin, Lin, Chan, Yang: "Antitumor effect of kurarinone and underlying mechanism in small cell lung carcinoma cells." in: OncoTargets and therapy, Vol. 12, pp. 6119-6131, (2019) (PubMed).

Lopez-Charcas, Espinosa, Alfaro, Herrera-Carrillo, Ramirez-Cordero, Cortes-Reynosa, Perez Salazar, Berumen, Gomora: "The invasiveness of human cervical cancer associated to the function of NaV1.6 channels is mediated by MMP-2 activity." in: Scientific reports, Vol. 8, Issue 1, pp. 12995, (2019) (PubMed).

Kim, Choi, Lee, Soh, Kim, Park: "Senescent tumor cells lead the collective invasion in thyroid cancer." in: Nature communications, Vol. 8, pp. 15208, (2018) (PubMed).

Gao, Zhao, Zhang, Lu, Liu, Geng, Huang, Zhang, Lu: "The dual function of PRMT1 in modulating epithelial-mesenchymal transition and cellular senescence in breast cancer cells through regulation of ZEB1." in: Scientific reports, Vol. 6, pp. 19874, (2016) (PubMed).

Target Details for MMP 9

Target: MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))

Alternative Name: matrix metallopeptidase 9

Background: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.

Cellular Localization: Secreted , extracellular space , extracellular matrix

Molecular Weight: 78 kDa

Gene ID: 4318

UniProt: P14780

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events