Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial (MCAD) (N-Term) antibody

Catalog No. ABIN2854768

This Rabbit Polyclonal antibody specifically detects in WB, IF, IHC (p) and ICC. It exhibits reactivity toward Human and Mouse.

Quick Overview for Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial (MCAD) (N-Term) antibody (ABIN2854768)

Target: Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial (MCAD)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details

Binding Specificity: N-Term

Cross-Reactivity: Cow (Bovine), Pig (Porcine)

Cross-Reactivity (Details): Mouse (81 %), Pig (83 %), Bovine (88 %)

Characteristics: Rabbit Polyclonal antibody to ACADM (acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain)
ACADM antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human ACADM. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: 293T , A431 , HeLa , HepG2 , Raji

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.74 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details

Target: Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial (MCAD)

Alternative Name: ACADM

Background: This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Cellular Localization: Mitochondrion matrix

Molecular Weight: 47 kDa

Gene ID: 34