Periostin antibody (C-Term)

Catalog No. ABIN2854805

The Rabbit Polyclonal anti-Periostin antibody has been validated for WB, ICC, IF, IHC (p) and IHC (fro). It is suitable to detect Periostin in samples from Human. There are 3+ publications available.

Quick Overview for Periostin antibody (C-Term) (ABIN2854805)

Target: Periostin (POSTN)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Periostin antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Periostin Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to Periostin (periostin, osteoblast specific factor)
Periostin antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human Periostin. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: H1299

Validation: KO/KD, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.29 mg/mL

Buffer: 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7), 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-Periostin antibody (ABIN2854805)

Lee, Yeh, Lee, Shih, Chen, Hung, You, Wu, Shentu, Huang, Lin, Wu, Lin, Lu, Tsao, Lin, Lo, Tseng, Wu, Chen, Wu, Lin, Sperling, Guzy, Fang, Yang: "Fibroblast-enriched endoplasmic reticulum protein TXNDC5 promotes pulmonary fibrosis by augmenting TGFβ signaling through TGFBR1 stabilization." in: Nature communications, Vol. 11, Issue 1, pp. 4254, (2020) (PubMed).

Nakazawa, Taniyama, Sanada, Morishita, Nakamori, Morimoto, Yeung, Yang: "Periostin blockade overcomes chemoresistance via restricting the expansion of mesenchymal tumor subpopulations in breast cancer." in: Scientific reports, Vol. 8, Issue 1, pp. 4013, (2019) (PubMed).

Fan, Jeng, Lu, Lien: "SPOCK1 Is a Novel Transforming Growth Factor-β-Induced Myoepithelial Marker That Enhances Invasion and Correlates with Poor Prognosis in Breast Cancer." in: PLoS ONE, Vol. 11, Issue 9, pp. e0162933, (2017) (PubMed).

Target Details for Periostin

Target: Periostin (POSTN)

Alternative Name: periostin

Background: Binds to heparin. Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization.

Cellular Localization: Secreted , extracellular space , extracellular matrix

Molecular Weight: 93 kDa

Gene ID: 10631

UniProt: Q15063