HDAC6 antibody (N-Term)

Catalog No. ABIN2854846

This Rabbit Polyclonal antibody specifically detects HDAC6 in WB, IF, IHC (p), ICC and FACS. It exhibits reactivity toward Human.

Quick Overview for HDAC6 antibody (N-Term) (ABIN2854846)

Target: HDAC6 (Histone Deacetylase 6 (HDAC6))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC6 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-HDAC6 Antibody

Binding Specificity: N-Term

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to HDAC6 (histone deacetylase 6)
HDAC6 antibody [N1], N-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human HDAC6. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 3xFlag-human HDAC6-transfected 293T

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.18 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for HDAC6

Target: HDAC6 (Histone Deacetylase 6 (HDAC6))

Alternative Name: histone deacetylase 6

Background: Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription.

Cellular Localization: Nucleus , Cytoplasm

Molecular Weight: 131 kDa

Gene ID: 10013

UniProt: Q9UBN7

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling