BMP4 antibody

Catalog No. ABIN2854883

The Rabbit Polyclonal anti-BMP4 antibody has been validated for WB, ICC, IF and IHC (p). It is suitable to detect BMP4 in samples from Human. There are 2+ publications available.

Quick Overview for BMP4 antibody (ABIN2854883)

Target: BMP4 (Bone Morphogenetic Protein 4 (BMP4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BMP4 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-BMP4 Antibody

Specificity: This antibody is able to recognize full length BMP4 protein (~55 kDa) and N-terminal propeptide BMP4 protein (~40 kDa) but unable to detect mature form.

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to BMP4 (bone morphogenetic protein 4)
BMP4 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human BMP4. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: NIH-3T3 , JC , HepG2 conditioned medium

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.36 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-BMP4 antibody (ABIN2854883)

Guo, Wang, Yuan, Zhu, Fu, Xu, Wu, Wu: "TSA restores hair follicle-inductive capacity of skin-derived precursors." in: Scientific reports, Vol. 9, Issue 1, pp. 2867, (2020) (PubMed).

Peng, Chen, Wang, Zhu, Wu, Luo, Zuo, Luo, Zhou, Shi, Weng, Huang, He, Fan: "Bone morphogenetic protein 4 (BMP4) alleviates hepatic steatosis by increasing hepatic lipid turnover and inhibiting the mTORC1 signaling axis in hepatocytes." in: Aging, Vol. 11, Issue 23, pp. 11520-11540, (2019) (PubMed).

Target Details for BMP4

Target: BMP4 (Bone Morphogenetic Protein 4 (BMP4))

Alternative Name: bone morphogenetic protein 4

Background: The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing in the 5' untranslated region of this gene has been described and three variants are described, all encoding an identical protein.

Cellular Localization: Secreted , extracellular space , extracellular matrix

Molecular Weight: 47 kDa

Gene ID: 652

UniProt: P12644

Pathways: Steroid Hormone Mediated Signaling Pathway, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development