PRNP antibody

Catalog No. ABIN2854915

This Rabbit Polyclonal antibody specifically detects PRNP in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for PRNP antibody (ABIN2854915)

Target: PRNP (Prion Protein (PRNP))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRNP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PRNP Antibody

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to PRP (prion protein)
PRP antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Prion Protein (PrP). The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Neuro2A , NIH-3T3 , C2C12 , IMR32

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.36 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for PRNP

Target: PRNP (Prion Protein (PRNP))

Alternative Name: prion protein

Background: The protein encoded by this gene is a membrane glycosylphosphatidylinositol-anchored glycoprotein that tends to aggregate into rod-like structures. The encoded protein contains a highly unstable region of five tandem octapeptide repeats. This gene is found on chromosome 20, approximately 20 kbp upstream of a gene which encodes a biochemically and structurally similar protein to the one encoded by this gene. Mutations in the repeat region as well as elsewhere in this gene have been associated with Creutzfeldt-Jakob disease, fatal familial insomnia, Gerstmann-Straussler disease, Huntington disease-like 1, and kuru. Alternative splicing results in multiple transcript variants encoding the same protein.

Cellular Localization: Cell membrane, Lipid-anchor , GPI-anchor , Golgi apparatus

Molecular Weight: 28 kDa

Gene ID: 5621

UniProt: P04156, F7VJQ1

Pathways: Transition Metal Ion Homeostasis, Activated T Cell Proliferation