ZAP70 antibody (Center)
Quick Overview for ZAP70 antibody (Center) (ABIN2854924)
Target
See all ZAP70 AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- Center
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Characteristics
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Rabbit Polyclonal antibody to ZAP70 (zeta-chain (TCR) associated protein kinase 70 kDa)
ZAP70 antibody -
Purification
- Purified by antigen-affinity chromatography.
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Immunogen
- Recombinant protein encompassing a sequence within the center region of human ZAP70. The exact sequence is proprietary.
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Isotype
- IgG
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Application Notes
- Suggested dilution Reference IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceIHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
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Comment
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Positive Control: Jurkat
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- 1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.
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Preservative
- ProClin
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Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C
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Storage Comment
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))
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Alternative Name
- ZAP70
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Background
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This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.
Cellular Localization: Cytoplasm (By similarity) -
Molecular Weight
- 70 kDa
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Gene ID
- 7535
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Pathways
- TCR Signaling, Ubiquitin Proteasome Pathway
Target
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