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ZAP70 antibody (Center)

This Rabbit Polyclonal antibody specifically detects ZAP70 in WB and IHC (p). It exhibits reactivity toward Human and Mouse.
Catalog No. ABIN2854924

Quick Overview for ZAP70 antibody (Center) (ABIN2854924)

Target

See all ZAP70 Antibodies
ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Reactivity

  • 255
  • 126
  • 49
  • 8
  • 7
  • 6
  • 6
  • 5
  • 5
  • 3
  • 3
  • 2
  • 1
Human, Mouse

Host

  • 190
  • 63
  • 2
  • 2
  • 1
Rabbit

Clonality

  • 182
  • 75
Polyclonal

Conjugate

  • 136
  • 13
  • 12
  • 9
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  • 4
  • 4
  • 4
  • 4
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  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
This ZAP70 antibody is un-conjugated

Application

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  • 22
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  • 12
  • 6
  • 6
  • 3
  • 2
  • 2
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • Binding Specificity

    • 34
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    • 25
    • 20
    • 17
    • 16
    • 10
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    • 8
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    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
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    • 1
    • 1
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    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Center

    Characteristics

    Rabbit Polyclonal antibody to ZAP70 (zeta-chain (TCR) associated protein kinase 70 kDa)
    ZAP70 antibody

    Purification

    Purified by antigen-affinity chromatography.

    Immunogen

    Recombinant protein encompassing a sequence within the center region of human ZAP70. The exact sequence is proprietary.

    Isotype

    IgG
  • Application Notes

    Suggested dilution Reference IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceIHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

    Comment

    Positive Control: Jurkat

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.

    Preservative

    ProClin

    Precaution of Use

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Target

    ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

    Alternative Name

    ZAP70

    Background

    This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.

    Cellular Localization: Cytoplasm (By similarity)

    Molecular Weight

    70 kDa

    Gene ID

    7535

    Pathways

    TCR Signaling, Ubiquitin Proteasome Pathway
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