HSP27 antibody (Center)

Catalog No. ABIN2854951

This Rabbit Polyclonal antibody specifically detects HSP27 in WB, IF, FACS, IP, IHC (p) and ICC. It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 2+ publications.

Quick Overview for HSP27 antibody (Center) (ABIN2854951)

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSP27 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-HSP27 Antibody

Binding Specificity: Center

Cross-Reactivity: Rhesus Monkey

Cross-Reactivity (Details): Rhesus Monkey (100 %)

Characteristics: Rabbit Polyclonal antibody to HSP27 (heat shock 27 kDa protein 1)
HSP27 antibody

Purification: Affinity purified by Protein A.

Immunogen: Recombinant protein encompassing a sequence within the center region of human HSP27. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference Flow cytometry/FACS Assay-dependent dilution ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:1000* Western blot 1:500-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceFlow cytometry/FACSAssay-dependent dilution ICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:1000* Western blot1:500-1:10000*

Comment:

Positive Control: A431 , HeLa , mouse liver , *HT29

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 3.23 mg/mL

Buffer: 1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-HSP27 antibody (ABIN2854951)

Cheung, Hsu, Lin, Chen, Wang, Huang, Juan: "ZNF322A-mediated protein phosphorylation induces autophagosome formation through modulation of IRS1-AKT glucose uptake and HSP-elicited UPR in lung cancer." in: Journal of biomedical science, Vol. 27, Issue 1, pp. 75, (2020) (PubMed).

Xu, Wu, Zheng, Yu, Yang, Yao, Zhou, Ching, Lau: "Proteome profiling of cadmium-induced apoptosis by antibody array analyses in human bronchial epithelial cells." in: Oncotarget, Vol. 7, Issue 5, pp. 6146-58, (2017) (PubMed).

Target Details for HSP27

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Alternative Name: HSP27

Background: The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).

Cellular Localization: Cytoplasm , Nucleus , Spindle

Molecular Weight: 23 kDa

Gene ID: 3315

Pathways: MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling