DLD antibody

Catalog No. ABIN2854985

The Rabbit Polyclonal anti-DLD antibody has been validated for WB, IF, ICC and IHC (p). It is suitable to detect DLD in samples from Human. There is 1 publication available.

Quick Overview for DLD antibody (ABIN2854985)

Target: DLD (Dihydrolipoamide Dehydrogenase (DLD))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DLD antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: KO Validated

Product Details anti-DLD Antibody

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to DLD (dihydrolipoamide dehydrogenase)
DLD antibody

Purification: Affinity purified by Protein A.

Immunogen: Recombinant protein encompassing a sequence within the center region of human DLD. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Mouse brain , 293T , PC-12 , Rat2 , HeLa

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-DLD antibody (ABIN2854985)

Shin, Lee, You, Kim, Roh: "Dihydrolipoamide dehydrogenase regulates cystine deprivation-induced ferroptosis in head and neck cancer." in: Redox biology, Vol. 30, pp. 101418, (2020) (PubMed).

Target Details for DLD

Target: DLD (Dihydrolipoamide Dehydrogenase (DLD))

Alternative Name: dihydrolipoamide dehydrogenase

Background: This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency.

Cellular Localization: Mitochondrion matrix

Molecular Weight: 54 kDa

Gene ID: 1738

UniProt: P09622

Pathways: Ribonucleoside Biosynthetic Process, Cell RedoxHomeostasis