Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial (MCAD) (Center) antibody

Catalog No. ABIN2855029

This Rabbit Polyclonal antibody specifically detects in WB, IF, IHC (p) and ICC. It exhibits reactivity toward Human and Mouse.

Quick Overview for Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial (MCAD) (Center) antibody (ABIN2855029)

Target: Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial (MCAD)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details

Binding Specificity: Center

Cross-Reactivity: Chimpanzee

Cross-Reactivity (Details): Chimpanzee (100 %)

Characteristics: Rabbit Polyclonal antibody to ACADM (acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain)
ACADM antibody

Purification: Affinity purified by Protein A.

Immunogen: Recombinant protein encompassing a sequence within the center region of human ACADM. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: A431 , NIH-3T3

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10.00 mg/mL

Buffer: 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details

Target: Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial (MCAD)

Alternative Name: ACADM

Background: This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Cellular Localization: Mitochondrion matrix

Molecular Weight: 47 kDa

Gene ID: 34