RPS27A antibody

Catalog No. ABIN2855033

The Rabbit Polyclonal anti-RPS27A antibody has been validated for IF and ICC. It is suitable to detect RPS27A in samples from Human.

Quick Overview for RPS27A antibody (ABIN2855033)

Target: RPS27A (Ribosomal Protein S27a (RPS27A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPS27A antibody is un-conjugated

Application: Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-RPS27A Antibody

Cross-Reactivity: Human

Characteristics: Rabbit Polyclonal antibody to RPS27A (ribosomal protein S27a)
RPS27A antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human RPS27A. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.4 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for RPS27A

Target: RPS27A (Ribosomal Protein S27a (RPS27A))

Alternative Name: ribosomal protein S27a

Background: Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin, similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.

Molecular Weight: 18 kDa

Gene ID: 6233

UniProt: P62979

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, Toll-Like Receptors Cascades, Synthesis of DNA, EGFR Downregulation