SAFB antibody

Catalog No. ABIN2855048

The Rabbit Polyclonal anti-SAFB antibody has been validated for WB, IF, IHC (p), ICC. It is suitable to detect SAFB in samples from Human.

Quick Overview for SAFB antibody (ABIN2855048)

Target: SAFB (Scaffold Attachment Factor B (SAFB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SAFB antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-SAFB Antibody

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit polyclonal antibody to Scaffold attachment factor B1 (scaffold attachment factor B)
Scaffold attachment factor B1 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human SAFB. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , A431 , HeLa , HepG2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for SAFB

Target: SAFB (Scaffold Attachment Factor B (SAFB))

Alternative Name: scaffold attachment factor B

Background: This gene encodes a DNA-binding protein that has high specificity for scaffold or matrix attachment region DNA elements (S/MAR DNA). This protein is thought to be involved in attaching the base of chromatin loops to the nuclear matrix but there is conflicting evidence as to whether this protein is a component of chromatin or a nuclear matrix protein. Scaffold attachment factors are a specific subset of nuclear matrix proteins (NMP) that specifically bind to S/MAR. This encoded protein is thought to serve as a molecular base to assemble a 'transcriptosome complex' in the vicinity of actively transcribed genes. It is involved in the regulation of the heat shock protein 27 transcription and also can act as an estrogen receptor corepressor. This gene is a candidate gene for breast tumorigenesis.

Cellular Localization: Nucleus

Molecular Weight: 103 kDa

Gene ID: 6294

UniProt: Q15424

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway