EIF4A2 antibody (N-Term)

Catalog No. ABIN2855061

This anti-EIF4A2 antibody is a Rabbit Polyclonal antibody detecting EIF4A2 in WB. Suitable for Human.

Quick Overview for EIF4A2 antibody (N-Term) (ABIN2855061)

Target: EIF4A2 (Eukaryotic Translation Initiation Factor 4A2 (EIF4A2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4A2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EIF4A2 Antibody

Binding Specificity: N-Term

Specificity: Based on sequence homology, this antibody may cross react with EIF4A1 or EIF4A3 protein.

Cross-Reactivity: Human

Characteristics: Rabbit polyclonal antibody to eIF4A2 (eukaryotic translation initiation factor 4A, isoform 2)
EIF4A2 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human EIF4A2. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T, A431

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.23 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for EIF4A2

Target: EIF4A2 (Eukaryotic Translation Initiation Factor 4A2 (EIF4A2))

Alternative Name: eukaryotic translation initiation factor 4A2

Background: ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

Molecular Weight: 46 kDa

Gene ID: 1974

UniProt: Q14240