COX5B antibody (Center)

Catalog No. ABIN2855062

This Rabbit Polyclonal antibody specifically detects COX5B in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for COX5B antibody (Center) (ABIN2855062)

Target: COX5B (Cytochrome C Oxidase Subunit Vb (COX5B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COX5B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-COX5B Antibody

Binding Specificity: Center

Cross-Reactivity: Mouse (Murine), Dog (Canine), Pig (Porcine), Rat (Rattus), Cow (Bovine)

Cross-Reactivity (Details): Mouse (85 %), Dog (83 %), Pig (83 %), Rat (82 %), Bovine (84 %)

Characteristics: Rabbit Polyclonal antibody to COX5B (cytochrome c oxidase subunit Vb)
COX5B antibody [N1C3]

Purification: Affinity purified by Protein A.

Immunogen: Recombinant protein encompassing a sequence within the center region of human COX5B. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceIHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: HepG2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.00 mg/mL

Buffer: 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for COX5B

Target: COX5B (Cytochrome C Oxidase Subunit Vb (COX5B))

Alternative Name: COX5B

Background: Cytochrome C oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Vb of the human mitochondrial respiratory chain enzyme.

Molecular Weight: 14 kDa

Gene ID: 1329