KLF4 antibody (C-Term)

Catalog No. ABIN2855075

The Rabbit Polyclonal anti-KLF4 antibody has been validated for WB, FACS and IP. It is suitable to detect KLF4 in samples from Human and Mouse. There is 1 publication available.

Quick Overview for KLF4 antibody (C-Term) (ABIN2855075)

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KLF4 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)

Product Details anti-KLF4 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Rat (Rattus), Cat (Feline), Pig (Porcine), Sheep (Ovine), Rhesus Monkey, Cow (Bovine)

Cross-Reactivity (Details): Rat (100 %), Cat (100 %), Pig (100 %), Sheep (100 %), Rhesus Monkey (100 %), Bovine (100 %)

Characteristics: Rabbit Polyclonal antibody to KLF4 (Kruppel-like factor 4 (gut))
KLF4 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human KLF4. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference Flow cytometry/FACS 1:50-1:200* Immunoprecipitation 1:100-1:500* Western blot 1:1000-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceFlow cytometry/FACS1:50-1:200* Immunoprecipitation1:100-1:500* Western blot1:1000-1:10000*

Comment:

Positive Control: A549 , HeLa , HepG2 , HCT116 , NIH-3T3 , human ESC , mouse ESC , HCT116 , HCT116 nuclear extract , *MDM-MB-231

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-KLF4 antibody (ABIN2855075)

Nishimura, Okamoto, Shibue, Mizuta, Shibayama, Yoshino, Murakami, Yamaguchi, Tanaka, Toida, Igarashi: "KLF4 is required for suppression of histamine synthesis by polyamines during bone marrow-derived mast cell differentiation." in: PLoS ONE, Vol. 15, Issue 2, pp. e0229744, (2020) (PubMed).

Target Details for KLF4

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Alternative Name: KLF4

Background: Transcription factor which acts as both an activator and repressor. Binds the CACCC core sequence. Binds to multiple sites in the 5'-flanking region of its own gene and can activate its own transcription. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development.

Cellular Localization: Nucleus

Molecular Weight: 55 kDa

Gene ID: 9314

Pathways: Peptide Hormone Metabolism, Stem Cell Maintenance