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Creatine Kinase, Brain (CKB) (Center) antibody Primary Antibody

CKB Reactivity: Human, Mouse, Rat ICC, IF, IHC (p), WB Host: Rabbit Polyclonal
Catalog No. ABIN2855158
$509.08
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100 μL
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  • Target
    Binding Specificity
    Center
    Reactivity
    Human, Mouse, Rat
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    Un-conjugated
    Application
    Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
    Cross-Reactivity
    Cow (Bovine), Chicken, Pig (Porcine), Rabbit, Xenopus laevis, Xenopus tropicalis
    Cross-Reactivity (Details)
    Chicken (97 %), Pig (96 %), Rabbit (96 %), Xenopus laevis (88 %), Bovine (96 %), Xenopus tropicalis (88 %)
    Characteristics
    Rabbit Polyclonal antibody to Creatine kinase (brain) (creatine kinase, brain)
    Creatine kinase (brain) antibody [N3C3]
    Purification
    Purified by antigen-affinity chromatography.
    Immunogen
    Recombinant protein encompassing a sequence within the center region of human Creatine kinase (brain). The exact sequence is proprietary.
    Isotype
    IgG
  • Application Notes
    Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:10000*
    Comment

    Positive Control: H1299 , HeLaS3 , mouse brain , rat brain

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.54 mg/mL
    Buffer
    0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
    Preservative
    Thimerosal (Merthiolate)
    Precaution of Use
    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Target
    Alternative Name
    Creatine Kinase (Brain) (CKB Antibody Abstract)
    Synonyms
    B-CK, CKBB, b-ck, ckbb, CKB, Bck, Ck-3, Ck3, Ckbb, bck, ckb, cb594, wu:fj36f10, wu:fj37d11, Ckbr, creatine kinase B, creatine kinase, brain L homeolog, creatine kinase B-type, creatine kinase, brain, creatine kinase, brain b, CKB, ckb.L, Ckb, ckb, ckbb
    Background
    The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized.

    Cellular Localization: Cytoplasm
    Molecular Weight
    43 kDa
    Gene ID
    1152
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