ENO1 antibody

Catalog No. ABIN2855176

This Rabbit Polyclonal antibody specifically detects ENO1 in WB, IF, ICC, IHC (p), DB and IHC (wm). It exhibits reactivity toward Human.

Quick Overview for ENO1 antibody (ABIN2855176)

Target: ENO1 (Enolase 1 (ENO1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ENO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Dot Blot (DB), Immunohistochemistry (Whole Mount) (IHC (wm))

Grade: KO Validated

Product Details anti-ENO1 Antibody

Cross-Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio)

Characteristics: Rabbit Polyclonal antibody to ENO1 (enolase 1, (alpha))
ENO1 antibody [N3C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human ENO1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Mouse brain , A431 , H1299 , 293T

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for ENO1

Target: ENO1 (Enolase 1 (ENO1))

Alternative Name: enolase 1

Background: This gene encodes one of three enolase isoenzymes found in mammals, it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome.

Cellular Localization: Cytoplasm , Cell membrane , myofibril , sarcomere , M-band , Isoform MBP-1: Nucleus

Molecular Weight: 47 kDa

Gene ID: 2023

UniProt: P06733