RPL5 antibody

Catalog No. ABIN2855187

This anti-RPL5 antibody is a Rabbit Polyclonal antibody detecting RPL5 in WB, IHC (p), IF and ICC. Suitable for Human. This Primary Antibody has been cited in 1 publication.

Quick Overview for RPL5 antibody (ABIN2855187)

Target: RPL5 (Ribosomal Protein L5 (RPL5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPL5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-RPL5 Antibody

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to RPL5 (ribosomal protein L5)
RPL5 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human RPL5. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Mouse brain , Rat2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.4 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-RPL5 antibody (ABIN2855187)

Chiang, Sparrer, van Gent, Lässig, Huang, Osterrieder, Hopfner, Gack: "Viral unmasking of cellular 5S rRNA pseudogene transcripts induces RIG-I-mediated immunity." in: Nature immunology, Vol. 19, Issue 1, pp. 53-62, (2017) (PubMed).

Target Details for RPL5

Target: RPL5 (Ribosomal Protein L5 (RPL5))

Alternative Name: ribosomal protein L5

Background: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L18P family of ribosomal proteins. It is located in the cytoplasm. The protein binds 5S rRNA to form a stable complex called the 5S ribonucleoprotein particle (RNP), which is necessary for the transport of nonribosome-associated cytoplasmic 5S rRNA to the nucleolus for assembly into ribosomes. The protein interacts specifically with the beta subunit of casein kinase II. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. This gene is co-transcribed with the small nucleolar RNA gene U21, which is located in its fifth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Cellular Localization: Cytoplasm , Nucleus , nucleolus

Molecular Weight: 34 kDa

Gene ID: 6125

UniProt: P46777