MIA3 antibody (N-Term)

Catalog No. ABIN2855189

The Rabbit Polyclonal anti-MIA3 antibody has been validated for WB, IHC (p), ICC and IF. It is suitable to detect MIA3 in samples from Human.

Quick Overview for MIA3 antibody (N-Term) (ABIN2855189)

Target: MIA3 (Melanoma Inhibitory Activity Family, Member 3 (MIA3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MIA3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-MIA3 Antibody

Binding Specificity: N-Term

Cross-Reactivity: Cow (Bovine)

Cross-Reactivity (Details): Bovine (89 %)

Characteristics: Rabbit Polyclonal antibody to MIA3 (melanoma inhibitory activity family, member 3)
MIA3 antibody [N1], N-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human MIA3. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 10 μg/mL* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)10 μg/mL* Western blot1:500-1:3000*

Comment:

Positive Control: A549 , HepG2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.30 mg/mL

Buffer: 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for MIA3

Target: MIA3 (Melanoma Inhibitory Activity Family, Member 3 (MIA3))

Alternative Name: MIA3

Background: Required for collagen VII (COL7A1) secretion by loading COL7A1 into transport carriers. May participate in cargo loading of COL7A1 at endoplasmic reticulum exit sites by binding to COPII coat subunits Sec23/24 and guiding SH3-bound COL7A1 into a growing carrier. Does not play a role in global protein secretion and is apparently specific to COL7A1 cargo loading. However, it may participate in secretion of other proteins in cells that do not secrete COL7A1.

Cellular Localization: Endoplasmic reticulum membrane, Single-pass type I membrane protein

Molecular Weight: 214 kDa

Gene ID: 375056