GAD antibody (N-Term)

Catalog No. ABIN2855214

This anti-GAD antibody is a Rabbit Polyclonal antibody detecting GAD in WB, IHC, ICC, IHC (p), IF, IHC (fro), IHC (ff) and IHC (wm). Suitable for Human.

Quick Overview for GAD antibody (N-Term) (ABIN2855214)

Target: GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAD antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Free Floating) (IHC (ff)), Immunohistochemistry (Whole Mount) (IHC (wm))

Product Details anti-GAD Antibody

Binding Specificity: N-Term

Specificity: This antibody is specific for GAD67 protein, and it does not cross-react with GAD65 protein.

Cross-Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio)

Characteristics: Rabbit Polyclonal antibody to GAD67 (glutamate decarboxylase 1 (brain, 67 kDa))
GAD67 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human GAD67. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: human GAD1-transfected 293T

Validation: Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.22 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for GAD

Target: GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Alternative Name: glutamate decarboxylase 1

Background: This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form.

Molecular Weight: 67 kDa

Gene ID: 2571

UniProt: Q99259