SLC32A1 antibody (N-Term)

Catalog No. ABIN2855225

The Rabbit Polyclonal anti-SLC32A1 antibody has been validated for WB, IHC (p) and mIHC and has been independently validated. It is suitable to detect SLC32A1 in samples from Human and Mouse.

Quick Overview for SLC32A1 antibody (N-Term) (ABIN2855225)

Target: SLC32A1 (Solute Carrier Family 32 (GABA Vesicular Transporter), Member 1 (SLC32A1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC32A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Multiplex Immunohistochemistry (mIHC)

Product Details anti-SLC32A1 Antibody

Binding Specificity: N-Term

Characteristics: Rabbit Polyclonal antibody to VGAT (solute carrier family 32 (GABA vesicular transporter), member 1)
VGAT antibody [N1N2], N-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human VGAT. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceIHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: U87-MG , mouse brain

Restrictions: For Research Use only

Independent Validation (mIHC)

Validation #104339 (Multiplex Immunohistochemistry)

by: Akoya Biosciences

No.: #104339

Date: 04/20/2021

Antigen: SLC32A1

Lot Number: 42838

Method validated: Multiplex Immunohistochemistry

Positive Control:

FFPE normal human cortex

Negative Control:

Unlabeled control (FFPE normal human cortex)

Notes:

Passed. The anti-SLC32A1 antibody ABIN2855225 produces punctate staining in close approximation with dendritic processes. This pattern is consistent with presynaptic staining, which is the expected site of SLC31A1 immunofluorescence.

Validation Images

FFPE normal human cortex tissue section labeled with anti-SLC32A1 antibody ABIN2855225 (green; bound to fluorophore AF488) after EDTA antigen retrieval. MAP2 and SYN1 were labeled with anti-MAP2 antibody ABIN125739 (red; bound to fluorophore ATTO 550) and anti-SYN1 antibody ABIN5542390 (blue; bound to fluorophore AF488).

Full Methods

Primary Antibody: ABIN2855225

Full Protocol:

• Protocol details are described in the Akoya Biosciences CODEX® User Manual (see https://www.akoyabio.com/wp-content/uploads/2021/01/CODEX-User-Manual.pdf).
• Tissue preparation as outlined in the Akoya Biosciences CODEX® User Manual fresh-frozen tissue protocol.
• Conjugation of the anti-SLC32A1 antibody ABIN2855225 to an oligo barcode used to bind oligo-conjugated fluorophore AF488.

Experimental Notes:

• No signal was detected in unlabeled specimens.

• Specific staining of SLC32A1 was observed with human FFPE cortical tissue sections with both citrate antigen retrieval and EDTA antigen retrieval.

• Optimal staining and signal to noise ratios were obtained if tissue was pre-treated for autofluorescence removal (see https://www.akoyabio.com/wp-content/uploads/2020/07/Customer-Demonstrated-Protocol-Autofluorescence-Quenching-Mar2020.pdf).

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for SLC32A1

Target: SLC32A1 (Solute Carrier Family 32 (GABA Vesicular Transporter), Member 1 (SLC32A1))

Alternative Name: VGAT

Background: The protein encoded by this gene is an integral membrane protein involved in gamma-aminobutyric acid (GABA) and glycine uptake into synaptic vesicles. The encoded protein is a member of amino acid/polyamine transporter family II.

Cellular Localization: Cytoplasmic vesicle membrane, Multi-pass membrane protein

Molecular Weight: 57 kDa

Gene ID: 140679

Pathways: Proton Transport