CYB5A antibody

Catalog No. ABIN2855226

The Rabbit Polyclonal anti-CYB5A antibody (ABIN2855226) specifically detects CYB5A in WB, IF and ICC. The antibody is reactive with Human samples.

Quick Overview for CYB5A antibody (ABIN2855226)

Target: CYB5A (Cytochrome B5 Type A (Microsomal) (CYB5A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYB5A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-CYB5A Antibody

Cross-Reactivity: Cat, Dog, Human

Characteristics: Rabbit polyclonal antibody to Cytochrome b5 (cytochrome b5 type A (microsomal))
Cytochrome b5 antibody [N1C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Cytochrome b5. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: CYB5A transfected 293T

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.28 mg/mL

Buffer: 1XPBS ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for CYB5A

Target: CYB5A (Cytochrome B5 Type A (Microsomal) (CYB5A))

Alternative Name: cytochrome b5 type A

Background: The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene.

Cellular Localization: Isoform 1: Endoplasmic reticulum membrane , Microsome membrane , Isoform 2: Cytoplasm

Molecular Weight: 15 kDa

Gene ID: 1528

UniProt: P00167