COBRA1 antibody (Center)

Catalog No. ABIN2855267

The Rabbit Polyclonal anti-COBRA1 antibody is suitable to detect COBRA1 in samples from Human and Mouse. It has been validated for WB, IF, IHC (p) and ICC.

Quick Overview for COBRA1 antibody (Center) (ABIN2855267)

Target: COBRA1 (Cofactor of BRCA1 (COBRA1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COBRA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-COBRA1 Antibody

Binding Specificity: Center

Characteristics: Rabbit Polyclonal antibody to COBRA1 (cofactor of BRCA1)
COBRA1 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human COBRA1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: H1299

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS, 40 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for COBRA1

Target: COBRA1 (Cofactor of BRCA1 (COBRA1))

Alternative Name: COBRA1

Background: NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2, MIM 606026), either NELFC or NELFD (TH1L, MIM 605297), and NELFE (RDBP, MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1, MIM 603555) and SPT5 (SUPT5H, MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]).[supplied by OMIM]

Cellular Localization: Nucleus

Molecular Weight: 66 kDa

Gene ID: 25920