PUF60 antibody (C-Term)

Catalog No. ABIN2855271

The Rabbit Polyclonal anti-PUF60 antibody (ABIN2855271) specifically detects PUF60 in WB, IHC (p), IF, ICC and IHC (wm). The antibody is reactive with Human samples.

Quick Overview for PUF60 antibody (C-Term) (ABIN2855271)

Target: PUF60 (Poly-U Binding Splicing Factor 60KDa (PUF60))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PUF60 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Whole Mount) (IHC (wm))

Product Details anti-PUF60 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human, Zebrafish (Danio rerio)

Characteristics: Rabbit Polyclonal antibody to PUF60 (poly-U binding splicing factor 60KDa)
PUF60 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human PUF60. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HepG2 , Molt-4 , Raji

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.81 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for PUF60

Target: PUF60 (Poly-U Binding Splicing Factor 60KDa (PUF60))

Alternative Name: poly(U) binding splicing factor 60

Background: The protein encoded by this gene is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. There are two alternatively spliced transcript variants of this gene encoding different isoforms. There seems to be evidence of multiple polyadenylation sites for this gene.

Cellular Localization: Nucleus

Molecular Weight: 60 kDa

Gene ID: 22827

UniProt: Q9UHX1