NXF1 antibody (C-Term)

Catalog No. ABIN2855276

The Rabbit Polyclonal anti-NXF1 antibody has been validated for WB, IP, IF and ICC. It is suitable to detect NXF1 in samples from Human.

Quick Overview for NXF1 antibody (C-Term) (ABIN2855276)

Target: NXF1 (Nuclear RNA Export Factor 1 (NXF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NXF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-NXF1 Antibody

Binding Specificity: C-Term

Sequence: CSSGALDYDI PTTASENLYF Q

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to TAP (nuclear RNA export factor 1)
TAP antibody [C2C3], C-term

Purification: Affinity purified by Protein A.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human TAP. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Mouse brain , A431

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10.00 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for NXF1

Target: NXF1 (Nuclear RNA Export Factor 1 (NXF1))

Alternative Name: nuclear RNA export factor 1

Background: This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. The encoded protein overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses. Alternative splicing results in multiple transcript variants.

Cellular Localization: Nucleus , nucleoplasm , Nucleus speckle , Cytoplasm

Molecular Weight: 70 kDa

Gene ID: 10482

UniProt: Q9UBU9