ADAMTSL2 antibody (C-Term)

Catalog No. ABIN2855279

The Rabbit Polyclonal anti-ADAMTSL2 antibody has been validated for WB and IHC (p). It is suitable to detect ADAMTSL2 in samples from Human. There are 2+ publications available.

Quick Overview for ADAMTSL2 antibody (C-Term) (ABIN2855279)

Target: ADAMTSL2 (ADAMTS-Like 2 (ADAMTSL2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADAMTSL2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ADAMTSL2 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to ADAMTSL2 (ADAMTS-like 2)
ADAMTSL2 antibody [C3], C-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human ADAMTSL2. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: H1299

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-ADAMTSL2 antibody (ABIN2855279)

Piccolo, Sabatino, Mithbaokar, Polishchuck, Law, Magraner-Pardo, Pons, Polishchuck, Brunetti-Pierri: "Geleophysic dysplasia: novel missense variants and insights into ADAMTSL2 intracellular trafficking." in: Molecular genetics and metabolism reports, Vol. 21, pp. 100504, (2019) (PubMed).

Aviram, Zaffryar-Eilot, Hubmacher, Grunwald, Mäki, Myllyharju, Apte, Hasson: "Interactions between lysyl oxidases and ADAMTS proteins suggest a novel crosstalk between two extracellular matrix families." in: Matrix biology : journal of the International Society for Matrix Biology, Vol. 75-76, pp. 114-125, (2019) (PubMed).

Target Details for ADAMTSL2

Target: ADAMTSL2 (ADAMTS-Like 2 (ADAMTSL2))

Alternative Name: ADAMTS like 2

Background: This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) and ADAMTS-like protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The protein encoded by this gene lacks the protease domain, and is therefore of a member of the the ADAMTS-like protein subfamily. It is a secreted glycoprotein that binds the cell surface and extracellular matrix, it also interacts with latent transforming growth factor beta binding protein 1. Mutations in this gene have been associated with geleophysic dysplasia.

Cellular Localization: Secreted

Molecular Weight: 105 kDa

Gene ID: 9719

UniProt: Q86TH1