PFN1 antibody

Catalog No. ABIN2855281

This Rabbit Polyclonal antibody specifically detects PFN1 in WB, ICC, IF and IHC (p). It exhibits reactivity toward Human.

Quick Overview for PFN1 antibody (ABIN2855281)

Target: PFN1 (Profilin 1 (PFN1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PFN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PFN1 Antibody

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to Profilin 1 (profilin 1)
Profilin 1 antibody [N1C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Profilin 1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , A431 , HeLa , HepG2 , mouse spleen , Rat spleen

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.56 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for PFN1

Target: PFN1 (Profilin 1 (PFN1))

Alternative Name: profilin 1

Background: The protein encoded by this gene is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals. Deletion of this gene is associated with Miller-Dieker syndrome.

Cellular Localization: Cytoplasm , cytoskeleton

Molecular Weight: 15 kDa

Gene ID: 5216

UniProt: P07737

Pathways: Regulation of Actin Filament Polymerization, Tube Formation, Maintenance of Protein Location