TUBA1B antibody

Catalog No. ABIN2855283

This anti-TUBA1B antibody is a Rabbit Polyclonal antibody detecting TUBA1B in WB, IF, IHC (p), ICC and IHC (wm). Suitable for Human. This Primary Antibody has been cited in 3+ publications.

Quick Overview for TUBA1B antibody (ABIN2855283)

Target: TUBA1B (Tubulin, alpha 1B (TUBA1B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TUBA1B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Whole Mount) (IHC (wm))

Product Details anti-TUBA1B Antibody

Cross-Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio)

Characteristics: Rabbit Polyclonal antibody to alpha Tubulin (tubulin, alpha 1b)
alpha Tubulin antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human alpha Tubulin. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , A431 , HeLa , HepG2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.14 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-TUBA1B antibody (ABIN2855283)

Lee, Jhuang, Chen, Jeng, Yuan: "Paradoxical overexpression of MBNL2 in hepatocellular carcinoma inhibits tumor growth and invasion." in: Oncotarget, Vol. 7, Issue 40, pp. 65589-65601, (2018) (PubMed).

Chang, Liu, Chiu, Chen, Chuang, Chen, Hsu, Lu, Chiou, Chou, Chiu: "A 4-Phenoxyphenol Derivative Exerts Inhibitory Effects on Human Hepatocellular Carcinoma Cells through Regulating Autophagy and Apoptosis Accompanied by Downregulating α-Tubulin Expression." in: Molecules (Basel, Switzerland), Vol. 22, Issue 5, (2017) (PubMed).

Chen, Ma, Wang, Wang, Wang, Xiao, Wang, Tan, Guo: "Thrombin promotes fibronectin secretion by bone marrow mesenchymal stem cells via the protease-activated receptor mediated signalling pathways." in: Stem cell research & therapy, Vol. 5, Issue 2, pp. 36, (2015) (PubMed).

Target Details for TUBA1B

Target: TUBA1B (Tubulin, alpha 1B (TUBA1B))

Alternative Name: tubulin, alpha 1b

Background: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

Molecular Weight: 50 kDa

Gene ID: 10376

Pathways: Microtubule Dynamics, M Phase