FOXM1 antibody

Catalog No. ABIN2855309

The Rabbit Polyclonal anti-FOXM1 antibody has been validated for WB, IP, ChIP and IHC (p). It is suitable to detect FOXM1 in samples from Human. There are 5+ publications available.

Quick Overview for FOXM1 antibody (ABIN2855309)

Target: FOXM1 (Forkhead Box M1 (FOXM1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: KO Validated

Product Details anti-FOXM1 Antibody

Cross-Reactivity: Human

Characteristics: Rabbit Polyclonal antibody to FOXM1 (forkhead box M1)
FOXM1 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human FOXM1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: FOXM1-transfected 293T

Validation: KO/KD, Orthogonal, Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.12 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-FOXM1 antibody (ABIN2855309)

Manzione, Rombouts, Steklov, Pasquali, Sablina, Gelens, Qian, Bollen: "Co-regulation of the antagonistic RepoMan:Aurora-B pair in proliferating cells." in: Molecular biology of the cell, Vol. 31, Issue 6, pp. 419-438, (2020) (PubMed).

Huang, Zhang, Jiang, Zhang, Xiang, Ren: "FoxM1 Induced Paclitaxel Resistance via Activation of the FoxM1/PHB1/RAF-MEK-ERK Pathway and Enhancement of the ABCA2 Transporter." in: Molecular therapy oncolytics, Vol. 14, pp. 196-212, (2019) (PubMed).

Cohn, Feldman, Weil, Kublanovsky, Levy: "Chromatin associated SETD3 negatively regulates VEGF expression." in: Scientific reports, Vol. 6, pp. 37115, (2018) (PubMed).

Xie, Wu, Mack, Yang, Kim, Hubert, Flavahan, Chu, Bao, Rich: "CDC20 maintains tumor initiating cells." in: Oncotarget, Vol. 6, Issue 15, pp. 13241-54, (2016) (PubMed).

Sanders, Ross-Innes, Beraldi, Carroll, Balasubramanian: "Genome-wide mapping of FOXM1 binding reveals co-binding with estrogen receptor alpha in breast cancer cells." in: Genome biology, Vol. 14, Issue 1, pp. R6, (2014) (PubMed).

Target Details for FOXM1

Target: FOXM1 (Forkhead Box M1 (FOXM1))

Alternative Name: forkhead box M1

Background: Cellular Localization: Nucleus

Molecular Weight: 84 kDa

Gene ID: 2305

UniProt: Q08050

Pathways: Positive Regulation of Response to DNA Damage Stimulus