14-3-3 theta antibody (Center)

Catalog No. ABIN2855319

This anti-14-3-3 theta antibody is a Rabbit Polyclonal antibody detecting 14-3-3 theta in WB and IHC (p). Suitable for Human and Mouse.

Quick Overview for 14-3-3 theta antibody (Center) (ABIN2855319)

Target: 14-3-3 theta (YWHAQ)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 theta antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-14-3-3 theta Antibody

Binding Specificity: Center

Cross-Reactivity: Rat (Rattus), Rabbit, Cow (Bovine)

Cross-Reactivity (Details): Rat (100 %), Rabbit (100 %), Bovine (100 %)

Characteristics: Rabbit Polyclonal antibody to 14-3-3 theta (tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide)
14-3-3 theta antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human 14-3-3 theta. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:1000-1:30000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceIHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:1000-1:30000*

Comment:

Positive Control: 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.84 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for 14-3-3 theta

Target: 14-3-3 theta (YWHAQ)

Alternative Name: 14-3-3 theta

Background: This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99 % identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease.

Cellular Localization: Cytoplasm

Molecular Weight: 28 kDa

Gene ID: 10971

Pathways: Apoptosis, Myometrial Relaxation and Contraction