DDOST antibody (Center)

Catalog No. ABIN2855324

The Rabbit Polyclonal anti-DDOST antibody has been validated for WB. It is suitable to detect DDOST in samples from Human.

Quick Overview for DDOST antibody (Center) (ABIN2855324)

Target: DDOST (Dolichyl-diphosphooligosaccharide--Protein Glycosyltransferase (DDOST))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDOST antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DDOST Antibody

Binding Specificity: Center

Cross-Reactivity: Mouse (Murine), Chicken, Dog (Canine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio), Cow (Bovine), Xenopus tropicalis

Cross-Reactivity (Details): Mouse (96 %), Chicken (93 %), Dog (97 %), Rat (96 %), Xenopus laevis (90 %), Zebrafish (86 %), Bovine (97 %), Xenopus tropicalis (93 %)

Characteristics: Rabbit polyclonal antibody to OST48 (dolichyl-diphosphooligosaccharide-protein glycosyltransferase)
OST48 antibody [C1C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human OST48. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceWestern blot1:500-1:3000*

Comment:

Positive Control: A431 , H1299 , HeLa , HepG2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.94 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for DDOST

Target: DDOST (Dolichyl-diphosphooligosaccharide--Protein Glycosyltransferase (DDOST))

Alternative Name: OST48

Background: This gene encodes a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. The product of this gene is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia.

Cellular Localization: Endoplasmic reticulum membrane, Single-pass type I membrane protein

Molecular Weight: 51 kDa

Gene ID: 1650

Pathways: Cell RedoxHomeostasis